Article: article from journal or magazin.
Protection against cutaneous leishmaniasis in outbred vervet monkeys using a recombinant histone H1 antigen.
Journal of Infectious Diseases
Infection with Leishmania major parasites results in the development of cutaneous ulcerative lesions on the skin. We investigated the protective potential of a single, recombinant histone H1 antigen against cutaneous leishmaniasis in an outbred population of vervet monkeys, using Montanide adjuvant. Protection was assessed by challenging the animals with a mixture of vector sand fly salivary-gland lysate and a low dose of in vitro-derived parasites, thus more closely mimicking natural infection induced by L. major. The course of infection in immunized monkeys was compared with that of animals that had healed from a primary infection and were immune. The monkeys immunized with recombinant histone H1 showed a reduced development of lesion size, compared with controls. Our study therefore illustrates the potential use of histone H1 as a vaccine candidate against cutaneous leishmaniasis in humans.
Adjuvants, Immunologic, Animals, Animals, Outbred Strains, Antibodies, Protozoan/blood, Cercopithecus aethiops, Disease Models, Animal, Histones/administration & dosage, Histones/genetics, Humans, Hypersensitivity, Delayed, Leishmania major/pathogenicity, Leishmaniasis, Cutaneous/parasitology, Leishmaniasis, Cutaneous/prevention & control, Protozoan Vaccines/administration & dosage, Protozoan Vaccines/immunology, Psychodidae/parasitology, Recombinant Proteins/administration & dosage, Recombinant Proteins/genetics, Vaccination
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