Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing

Détails

Ressource 1Télécharger: Arpat_biorxv.pdf (18441.94 [Ko])
Etat: Public
Version: de l'auteur
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_071D4E7F8B17
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Compte-rendu: analyse d'une oeuvre publiée.
Collection
Publications
Titre
Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing
Périodique
bioRxiv
Auteur(s)
Arpat A.B., Liechti A., De Matos M., Dreos R., Janich P., Gatfield D.
Statut éditorial
Publié
Date de publication
2019
Langue
anglais
Résumé
Translation initiation is considered overall rate-limiting for protein biosynthesis, whereas the impact of non-uniform ribosomal elongation rates is largely unknown. Using a modified ribosome profiling protocol based on footprints from two closely packed ribosomes (disomes), we have mapped ribosomal collisions transcriptome-wide in mouse liver. We uncover that the stacking of an elongating onto a paused ribosome occurs frequently and scales with translation rate, trapping ∼10% of translating ribosomes in the disome state. A distinct class of pause sites, independent of translation rate, is indicative of deterministic pausing signals. Pause sites are associated with specific amino acids, peptide motifs, and with structural features of the nascent polypeptide, suggestive of programmed pausing as a widespread mechanism associated with protein folding. Evolutionary conservation at disome sites and experiments indicate functional relevance of translational pausing. Collectively, our disome profiling approach allows novel, unexpected insights into gene regulation occurring at the step of translation elongation.
Open Access
Oui
Création de la notice
12/08/2019 8:54
Dernière modification de la notice
13/02/2020 7:19
Données d'usage