Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing.

Détails

Ressource 1Télécharger: Preprint_accepted version (18441.94 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_071D4E7F8B17
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing.
Périodique
Genome research
Auteur⸱e⸱s
Arpat A.B., Liechti A., De Matos M., Dreos R., Janich P., Gatfield D.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Statut éditorial
Publié
Date de publication
07/2020
Peer-reviewed
Oui
Volume
30
Numéro
7
Pages
985-999
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Translation initiation is the major regulatory step defining the rate of protein production from an mRNA. Meanwhile, the impact of nonuniform ribosomal elongation rates is largely unknown. Using a modified ribosome profiling protocol based on footprints from two closely packed ribosomes (disomes), we have mapped ribosomal collisions transcriptome-wide in mouse liver. We uncover that the stacking of an elongating onto a paused ribosome occurs frequently and scales with translation rate, trapping ∼10% of translating ribosomes in the disome state. A distinct class of pause sites is indicative of deterministic pausing signals. Pause site association with specific amino acids, peptide motifs, and nascent polypeptide structure is suggestive of programmed pausing as a widespread mechanism associated with protein folding. Evolutionary conservation at disome sites indicates functional relevance of translational pausing. Collectively, our disome profiling approach allows unique insights into gene regulation occurring at the step of translation elongation.
Mots-clé
Amino Acids, Animals, Codon, Codon Usage, Evolution, Molecular, Mice, Peptide Chain Elongation, Translational, Peptides/chemistry, Protein Biosynthesis, Protein Sorting Signals, Protein Structure, Secondary, Ribosomes/metabolism, Sequence Analysis, RNA, Transcriptome
Pubmed
Web of science
Open Access
Oui
Financement(s)
Fonds national suisse
Création de la notice
12/08/2019 7:54
Dernière modification de la notice
21/11/2022 8:26
Données d'usage