Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing.

Details

Ressource 1Download: Preprint_accepted version (18441.94 [Ko])
State: Public
Version: Author's accepted manuscript
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_071D4E7F8B17
Type
Article: article from journal or magazin.
Publication sub-type
Minutes: analyse of a published work.
Collection
Publications
Institution
Title
Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing.
Journal
Genome research
Author(s)
Arpat A.B., Liechti A., De Matos M., Dreos R., Janich P., Gatfield D.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
07/2020
Peer-reviewed
Oui
Volume
30
Number
7
Pages
985-999
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Translation initiation is the major regulatory step defining the rate of protein production from an mRNA. Meanwhile, the impact of nonuniform ribosomal elongation rates is largely unknown. Using a modified ribosome profiling protocol based on footprints from two closely packed ribosomes (disomes), we have mapped ribosomal collisions transcriptome-wide in mouse liver. We uncover that the stacking of an elongating onto a paused ribosome occurs frequently and scales with translation rate, trapping ∼10% of translating ribosomes in the disome state. A distinct class of pause sites is indicative of deterministic pausing signals. Pause site association with specific amino acids, peptide motifs, and nascent polypeptide structure is suggestive of programmed pausing as a widespread mechanism associated with protein folding. Evolutionary conservation at disome sites indicates functional relevance of translational pausing. Collectively, our disome profiling approach allows unique insights into gene regulation occurring at the step of translation elongation.
Keywords
Genetics(clinical), Genetics
Pubmed
Open Access
Yes
Funding(s)
Swiss National Science Foundation
Create date
12/08/2019 7:54
Last modification date
12/08/2020 5:22
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