Article: article from journal or magazin.
TLR5 signaling stimulates the innate production of IL-17 and IL-22 by CD3(neg)CD127+ immune cells in spleen and mucosa.
Journal of Immunology
In adaptive immunity, Th17 lymphocytes produce the IL-17 and IL-22 cytokines that stimulate mucosal antimicrobial defenses and tissue repair. In this study, we observed that the TLR5 agonist flagellin induced swift and transient transcription of genes encoding IL-17 and IL-22 in lymphoid, gut, and lung tissues. This innate response also temporarily enhanced the expression of genes associated with the antimicrobial Th17 signature. The source of the Th17-related cytokines was identified as novel populations of CD3(neg)CD127(+) immune cells among which CD4-expressing cells resembling lymphoid tissue inducer cells. We also demonstrated that dendritic cells are essential for expression of Th17-related cytokines and so for stimulation of innate cells. These data define that TLR-induced activation of CD3(neg)CD127(+) cells and production of Th17-related cytokines may be crucial for the early defenses against pathogen invasion of host tissues.
Animals, Antigens, CD3/genetics, Antigens, CD3/immunology, Cells, Cultured, Dendritic Cells/drug effects, Dendritic Cells/immunology, Female, Flagellin/pharmacology, Flow Cytometry, Gene Expression/drug effects, Gene Expression/immunology, Ileum/drug effects, Ileum/immunology, Interleukin-17/genetics, Interleukin-17/immunology, Interleukin-7 Receptor alpha Subunit/genetics, Interleukin-7 Receptor alpha Subunit/immunology, Interleukins/genetics, Interleukins/immunology, Lymphoid Tissue/cytology, Lymphoid Tissue/immunology, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Mice, SCID, Mice, Transgenic, Mucous Membrane/cytology, Mucous Membrane/immunology, Oligonucleotide Array Sequence Analysis, Signal Transduction/immunology, Spleen/cytology, Spleen/immunology, Toll-Like Receptor 5/genetics, Toll-Like Receptor 5/immunology
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