Local shockwave-application prevents flap necrosis
Details
Serval ID
serval:BIB_FB72AACDFE31
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Local shockwave-application prevents flap necrosis
Title of the conference
ESSR 2010, 45th Congress of the European Society for Surgical Research
Address
Geneva, Switzerland, June 9-12, 2010
ISBN
0007-1323
Publication state
Published
Issued date
2010
Volume
97
Series
British Journal of Surgery
Pages
S16
Language
english
Notes
Meeting Abstract
Abstract
Objective:
Local shockwave-application (SW) has shown to improve healing
of various tissues and decrease necrosis of flaps. Though, there is no data about
the optimal time-point of SW-application with regard to induction of ischemia
(i.e. flap elevation) and subsequent effect on flap survival. Therefore we compared
2 shock-wave protocols in a model of persistent ischemia and investigated
underlying mechanisms.
Methods:
18 C57BL/6-mice equipped with a skinfold chamber containing a
musculocutaneous flap were assigned to 3 experimental groups: 1. One session
of 500 SWimpulses at 0·15 mJ/mm2 applied 24 hrs before (preconditioning) or
2. Applied 30 min after flap elevation (treatment). 3. Untreated flaps (control).
Tissue necrosis,microhemodynamics, inflammation, apoptosis and angiogenesis
were assessed by intravital epi-fluorescence microscopy over 10 days.
Results:
SW significantly reduced flap necrosis independent from the
application time-point (preconditioning: 29 ± 7%; treatment: 25 ± 7% vs.
control: 47 ± 2%; d10, p<0·05). This was associated with an early increase
of functional capillary density (preconditioning: 236 ± 39 cm/cm2; treatment:
211 ± 33 cm/cm2 vs. control: 141 ± 7 cm/cm2; day1, p<0·05). Arteriolar diameter,
red blood cell velocity and blood flow were comparable between the
3 experimental groups. SW-application significantly decreased the ischemiainduced
inflammatory response (apoptotic cell death and leukocyte-endothelial
interaction: (p<0·05)). Sprouts indicating angiogenesis were observed from day
7 only after SW-application.
Conclusions:
SW protects ischemically challenged musculocutaneous tissue.
Interestingly, postoperative SW-application is as efficient as preoperative SWapplication.
The protective effect induced by mechanical stress might be based
on an early recruitment of ''sleeping capillaries'' maintaining nutritive perfusion
and an anti-inflammatory effect within the ischemically jeopardized tissue. SWapplication
provides a non-invasive alternative to local thermic and systemic
pre-treatment of endangered tissues.
Local shockwave-application (SW) has shown to improve healing
of various tissues and decrease necrosis of flaps. Though, there is no data about
the optimal time-point of SW-application with regard to induction of ischemia
(i.e. flap elevation) and subsequent effect on flap survival. Therefore we compared
2 shock-wave protocols in a model of persistent ischemia and investigated
underlying mechanisms.
Methods:
18 C57BL/6-mice equipped with a skinfold chamber containing a
musculocutaneous flap were assigned to 3 experimental groups: 1. One session
of 500 SWimpulses at 0·15 mJ/mm2 applied 24 hrs before (preconditioning) or
2. Applied 30 min after flap elevation (treatment). 3. Untreated flaps (control).
Tissue necrosis,microhemodynamics, inflammation, apoptosis and angiogenesis
were assessed by intravital epi-fluorescence microscopy over 10 days.
Results:
SW significantly reduced flap necrosis independent from the
application time-point (preconditioning: 29 ± 7%; treatment: 25 ± 7% vs.
control: 47 ± 2%; d10, p<0·05). This was associated with an early increase
of functional capillary density (preconditioning: 236 ± 39 cm/cm2; treatment:
211 ± 33 cm/cm2 vs. control: 141 ± 7 cm/cm2; day1, p<0·05). Arteriolar diameter,
red blood cell velocity and blood flow were comparable between the
3 experimental groups. SW-application significantly decreased the ischemiainduced
inflammatory response (apoptotic cell death and leukocyte-endothelial
interaction: (p<0·05)). Sprouts indicating angiogenesis were observed from day
7 only after SW-application.
Conclusions:
SW protects ischemically challenged musculocutaneous tissue.
Interestingly, postoperative SW-application is as efficient as preoperative SWapplication.
The protective effect induced by mechanical stress might be based
on an early recruitment of ''sleeping capillaries'' maintaining nutritive perfusion
and an anti-inflammatory effect within the ischemically jeopardized tissue. SWapplication
provides a non-invasive alternative to local thermic and systemic
pre-treatment of endangered tissues.
Web of science
Create date
21/10/2010 11:39
Last modification date
20/08/2019 17:26
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