Local shockwave-application prevents flap necrosis

Détails

ID Serval
serval:BIB_FB72AACDFE31
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Local shockwave-application prevents flap necrosis
Titre de la conférence
ESSR 2010, 45th Congress of the European Society for Surgical Research
Auteur⸱e⸱s
Harder Y., Vigato E., Pittet B., Wettstein R., Rezaeian F., Tobalem M.
Adresse
Geneva, Switzerland, June 9-12, 2010
ISBN
0007-1323
Statut éditorial
Publié
Date de publication
2010
Volume
97
Série
British Journal of Surgery
Pages
S16
Langue
anglais
Notes
Meeting Abstract
Résumé
Objective:
Local shockwave-application (SW) has shown to improve healing
of various tissues and decrease necrosis of flaps. Though, there is no data about
the optimal time-point of SW-application with regard to induction of ischemia
(i.e. flap elevation) and subsequent effect on flap survival. Therefore we compared
2 shock-wave protocols in a model of persistent ischemia and investigated
underlying mechanisms.
Methods:
18 C57BL/6-mice equipped with a skinfold chamber containing a
musculocutaneous flap were assigned to 3 experimental groups: 1. One session
of 500 SWimpulses at 0·15 mJ/mm2 applied 24 hrs before (preconditioning) or
2. Applied 30 min after flap elevation (treatment). 3. Untreated flaps (control).
Tissue necrosis,microhemodynamics, inflammation, apoptosis and angiogenesis
were assessed by intravital epi-fluorescence microscopy over 10 days.
Results:
SW significantly reduced flap necrosis independent from the
application time-point (preconditioning: 29 ± 7%; treatment: 25 ± 7% vs.
control: 47 ± 2%; d10, p<0·05). This was associated with an early increase
of functional capillary density (preconditioning: 236 ± 39 cm/cm2; treatment:
211 ± 33 cm/cm2 vs. control: 141 ± 7 cm/cm2; day1, p<0·05). Arteriolar diameter,
red blood cell velocity and blood flow were comparable between the
3 experimental groups. SW-application significantly decreased the ischemiainduced
inflammatory response (apoptotic cell death and leukocyte-endothelial
interaction: (p<0·05)). Sprouts indicating angiogenesis were observed from day
7 only after SW-application.
Conclusions:
SW protects ischemically challenged musculocutaneous tissue.
Interestingly, postoperative SW-application is as efficient as preoperative SWapplication.
The protective effect induced by mechanical stress might be based
on an early recruitment of ''sleeping capillaries'' maintaining nutritive perfusion
and an anti-inflammatory effect within the ischemically jeopardized tissue. SWapplication
provides a non-invasive alternative to local thermic and systemic
pre-treatment of endangered tissues.
Web of science
Création de la notice
21/10/2010 10:39
Dernière modification de la notice
20/08/2019 16:26
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