Article: article from journal or magazin.
A Chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions.
Methods in Molecular Biology
Chromosome organization, DNA replication, and transcription are only some of the processes relying on dynamic and highly regulated protein-DNA interactions. Here, we describe a biochemical assay to study the molecular details of associations between ring-shaped protein complexes and chromosomes in the context of living cells. Any protein complex embracing chromosomal DNA can be enriched by this method, allowing for the underlying loading mechanisms to be investigated.
Agarose plug, BMOE, Beta-clamp, Chromosome organization, Cysteine cross-linking, DNA entrapment, HaloTag labeling, Smc-ScpAB
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