A Chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions.
Détails
ID Serval
serval:BIB_F71D641D5A42
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Institution
Titre
A Chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions.
Titre du livre
Methods in molecular biology
Editeur
Springer
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
1624
Numéro de chapitre
10
Pages
117-126
Langue
anglais
Résumé
Chromosome organization, DNA replication, and transcription are only some of the processes relying on dynamic and highly regulated protein-DNA interactions. Here, we describe a biochemical assay to study the molecular details of associations between ring-shaped protein complexes and chromosomes in the context of living cells. Any protein complex embracing chromosomal DNA can be enriched by this method, allowing for the underlying loading mechanisms to be investigated.
Mots-clé
Bacillus subtilis/genetics, Bacillus subtilis/metabolism, Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, Binding Sites, Chromosomes, Bacterial/chemistry, Chromosomes, Bacterial/metabolism, Cross-Linking Reagents, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/metabolism, Protein Binding, Agarose plug, BMOE, Beta-clamp, Chromosome organization, Cysteine cross-linking, DNA entrapment, HaloTag labeling, Smc-ScpAB
Pubmed
Création de la notice
12/09/2017 13:53
Dernière modification de la notice
30/07/2024 6:02
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