A Chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions.

Details

Serval ID
serval:BIB_F71D641D5A42
Type
Article: article from journal or magazin.
Collection
Publications
Title
A Chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions.
Journal
Methods in molecular biology
Author(s)
Wilhelm L., Gruber S.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
1624
Pages
117-126
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Chromosome organization, DNA replication, and transcription are only some of the processes relying on dynamic and highly regulated protein-DNA interactions. Here, we describe a biochemical assay to study the molecular details of associations between ring-shaped protein complexes and chromosomes in the context of living cells. Any protein complex embracing chromosomal DNA can be enriched by this method, allowing for the underlying loading mechanisms to be investigated.

Keywords
Bacillus subtilis/genetics, Bacillus subtilis/metabolism, Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, Binding Sites, Chromosomes, Bacterial/chemistry, Chromosomes, Bacterial/metabolism, Cross-Linking Reagents, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/metabolism, Protein Binding, Agarose plug, BMOE, Beta-clamp, Chromosome organization, Cysteine cross-linking, DNA entrapment, HaloTag labeling, Smc-ScpAB
Pubmed
Create date
12/09/2017 14:53
Last modification date
20/08/2019 17:23
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