A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis

Détails

ID Serval
serval:BIB_EEBF69E055C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis
Périodique
FEMS Microbiology Letters
Auteur(s)
Mueller  C., Hutter  P., Sahli  R., Bretz  A., Toutoungi  L. N., Peter  O.
ISSN
0378-1097 (Print)
Statut éditorial
Publié
Date de publication
10/2000
Volume
191
Numéro
2
Pages
199-203
Notes
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Résumé
Borrelia garinii is one of the three major Borreliae responsible for Lyme borreliosis in Europe. We have characterized a protein of B. garinii (VS102) and a genomic fragment from the gene encoding this protein was cloned. The DNA sequence of the fragment showed high homology with a known gene of B. burgdorferi sensu stricto. The protein encoded by this gene in B. burgdorferi sensu stricto is a phosphocarrier protein (histidine-containing protein). A mutation T to G polymorphism at codon 57 was found to be specific to B. garinii. A PCR-based approach that allows the rapid detection of this mutation made it possible to specifically discriminate B. garinii from other B. burgdorferi genospecies with high sensitivity and specificity.
Mots-clé
Animals *Bacterial Proteins Base Sequence Borrelia/*classification/genetics/isolation & purification Cloning, Molecular Genes, Bacterial Humans Lyme Disease/*microbiology Molecular Sequence Data Mutation Phosphoenolpyruvate Sugar Phosphotransferase System/*genetics Polymerase Chain Reaction/*methods Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 21:51
Dernière modification de la notice
09/05/2019 3:10
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