Glucocorticoid modulation of plasminogen activators and of one of their inhibitors in the human mammary carcinoma cell line MDA-MB-231

Details

Serval ID
serval:BIB_E4A8797F290B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Glucocorticoid modulation of plasminogen activators and of one of their inhibitors in the human mammary carcinoma cell line MDA-MB-231
Journal
Cancer Research
Author(s)
Busso  N., Belin  D., Failly-Crepin  C., Vassalli  J. D.
ISSN
0008-5472 (Print)
Publication state
Published
Issued date
01/1987
Volume
47
Number
2
Pages
364-70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Abstract
In cultures of the human mammary carcinoma-derived cell line MDA-MB-231, plasminogen activator (PA) activity was reduced substantially following treatment with the glucocorticoid dexamethasone. These cells produced urokinase-type PA (u-PA) and tissue-type PA (t-PA), and both enzymes were decreased in dexamethasone-treated cultures. The drop in u-PA activity was associated with a decrease in the synthesis of single-chain pro-u-PA and in the concentration of u-PA messenger RNA; however, the decrease in u-PA activity was more extensive than could be accounted for by inhibition of enzyme synthesis only, suggesting that postsynthetic events were also involved. The comparatively small dexamethasone-induced decrease in t-PA activity was not associated with a change in the concentration of t-PA messenger RNA. Hence, the two PA genes are differentially regulated by the same hormone. MDA-MB-231 cells also produced a PA-specific inhibitor related to that produced by bovine aortic endothelial cells (PAI-1). This inhibitor was present in two forms: one functionally active, and the other which required activation by sodium dodecyl sulfate; both forms were increased in cultures exposed to dexamethasone. Thus, glucocorticoid-induced inhibition of PA activity in these cells results from a decrease in u-PA synthesis and a concomitant increase in the production of a PA inhibitor.
Keywords
Breast Neoplasms/*enzymology Cell Line Dexamethasone/*pharmacology Gene Expression Regulation/drug effects Humans Plasminogen Activators/antagonists & inhibitors/genetics/*metabolism Plasminogen Inactivators Protease Inhibitors/genetics RNA, Messenger/genetics Tissue Plasminogen Activator/genetics/metabolism Urinary Plasminogen Activator/genetics/metabolism
Pubmed
Web of science
Create date
25/01/2008 8:29
Last modification date
20/08/2019 16:08
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