Glucocorticoid modulation of plasminogen activators and of one of their inhibitors in the human mammary carcinoma cell line MDA-MB-231
Détails
ID Serval
serval:BIB_E4A8797F290B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Glucocorticoid modulation of plasminogen activators and of one of their inhibitors in the human mammary carcinoma cell line MDA-MB-231
Périodique
Cancer Research
ISSN
0008-5472 (Print)
Statut éditorial
Publié
Date de publication
01/1987
Volume
47
Numéro
2
Pages
364-70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Résumé
In cultures of the human mammary carcinoma-derived cell line MDA-MB-231, plasminogen activator (PA) activity was reduced substantially following treatment with the glucocorticoid dexamethasone. These cells produced urokinase-type PA (u-PA) and tissue-type PA (t-PA), and both enzymes were decreased in dexamethasone-treated cultures. The drop in u-PA activity was associated with a decrease in the synthesis of single-chain pro-u-PA and in the concentration of u-PA messenger RNA; however, the decrease in u-PA activity was more extensive than could be accounted for by inhibition of enzyme synthesis only, suggesting that postsynthetic events were also involved. The comparatively small dexamethasone-induced decrease in t-PA activity was not associated with a change in the concentration of t-PA messenger RNA. Hence, the two PA genes are differentially regulated by the same hormone. MDA-MB-231 cells also produced a PA-specific inhibitor related to that produced by bovine aortic endothelial cells (PAI-1). This inhibitor was present in two forms: one functionally active, and the other which required activation by sodium dodecyl sulfate; both forms were increased in cultures exposed to dexamethasone. Thus, glucocorticoid-induced inhibition of PA activity in these cells results from a decrease in u-PA synthesis and a concomitant increase in the production of a PA inhibitor.
Mots-clé
Breast Neoplasms/*enzymology
Cell Line
Dexamethasone/*pharmacology
Gene Expression Regulation/drug effects
Humans
Plasminogen Activators/antagonists & inhibitors/genetics/*metabolism
Plasminogen Inactivators
Protease Inhibitors/genetics
RNA, Messenger/genetics
Tissue Plasminogen Activator/genetics/metabolism
Urinary Plasminogen Activator/genetics/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 9:29
Dernière modification de la notice
20/08/2019 17:08