Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.

Détails

Ressource 1Télécharger: PIIS0092867415014853.pdf (4041.29 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_E29E6F7F6BE0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.
Périodique
Cell
Auteur(s)
Gouge J., Satia K., Guthertz N., Widya M., Thompson A.J., Cousin P., Dergai O., Hernandez N., Vannini A.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
2015
Volume
163
Numéro
6
Pages
1375-1387
Langue
anglais
Résumé
TFIIB-related factor 2 (Brf2) is a member of the family of TFIIB-like core transcription factors. Brf2 recruits RNA polymerase (Pol) III to type III gene-external promoters, including the U6 spliceosomal RNA and selenocysteine tRNA genes. Found only in vertebrates, Brf2 has been linked to tumorigenesis but the underlying mechanisms remain elusive. We have solved crystal structures of a human Brf2-TBP complex bound to natural promoters, obtaining a detailed view of the molecular interactions occurring at Brf2-dependent Pol III promoters and highlighting the general structural and functional conservation of human Pol II and Pol III pre-initiation complexes. Surprisingly, our structural and functional studies unravel a Brf2 redox-sensing module capable of specifically regulating Pol III transcriptional output in living cells. Furthermore, we establish Brf2 as a central redox-sensing transcription factor involved in the oxidative stress pathway and provide a mechanistic model for Brf2 genetic activation in lung and breast cancer.
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/01/2016 10:59
Dernière modification de la notice
09/05/2019 2:31
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