Liver X receptor preferentially activates de novo lipogenesis in human preadipocytes
Details
Serval ID
serval:BIB_E039F552E458
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Liver X receptor preferentially activates de novo lipogenesis in human preadipocytes
Journal
Biochimie
ISSN
0300-9084 (Print)
Publication state
Published
Issued date
04/2006
Volume
88
Number
3-4
Pages
309-18
Notes
Journal Article --- Old month value: Mar-Apr
Abstract
The liver X receptor (LXR) was demonstrated to play a key role in cholesterol metabolism in liver, intestine and macrophage. However, its function on the regulation of preadipocyte differentiation remains unclear since contradictory results were reported. The objective of the present study was to unravel the functionality of LXR in human preadipocytes. We show that the LXR agonist T0901317 strongly stimulated the expression of SREBP-1c and the lipogenic enzymes ACC-1, FAS and SCD-1 in both the human preadipose cell line Chub-S7 as well as human primary stromal vascular fraction (SVF) cells. The effects on gene expression were associated with the stimulation of de novo lipogenesis in both cell models, resulting in the induction of lipid accumulation. In contrast with a PPARgamma agonist (BRL49653), T0901317 enhanced only slightly the expression of PPARgamma dependent genes (PPARgamma, aP2 and adiponectin) in Chub-S7 cells and failed to change their expression in human SVF cells. These results show that LXR stimulated preferentially triglyceride accumulation in human preadipocytes via the induction of de novo lipogenesis, rather than activating the differentiation process through PPARgamma activation.
Keywords
Adipocytes/cytology/*metabolism
Biological Markers
Cell Differentiation
Cells, Cultured
DNA-Binding Proteins/*metabolism/pharmacology
Gene Expression Regulation
Humans
*Lipogenesis
PPAR gamma/metabolism
Receptors, Cytoplasmic and Nuclear/*metabolism
Stem Cells/*metabolism
Stromal Cells/metabolism
Transcription Factors/metabolism
Triglycerides/biosynthesis/metabolism
Pubmed
Web of science
Create date
25/01/2008 17:23
Last modification date
20/08/2019 17:04