Liver X receptor preferentially activates de novo lipogenesis in human preadipocytes

Détails

ID Serval
serval:BIB_E039F552E458
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Liver X receptor preferentially activates de novo lipogenesis in human preadipocytes
Périodique
Biochimie
Auteur(s)
Darimont  C., Avanti  O., Zbinden  I., Leone-Vautravers  P., Mansourian  R., Giusti  V., Mace  K.
ISSN
0300-9084 (Print)
Statut éditorial
Publié
Date de publication
04/2006
Volume
88
Numéro
3-4
Pages
309-18
Notes
Journal Article --- Old month value: Mar-Apr
Résumé
The liver X receptor (LXR) was demonstrated to play a key role in cholesterol metabolism in liver, intestine and macrophage. However, its function on the regulation of preadipocyte differentiation remains unclear since contradictory results were reported. The objective of the present study was to unravel the functionality of LXR in human preadipocytes. We show that the LXR agonist T0901317 strongly stimulated the expression of SREBP-1c and the lipogenic enzymes ACC-1, FAS and SCD-1 in both the human preadipose cell line Chub-S7 as well as human primary stromal vascular fraction (SVF) cells. The effects on gene expression were associated with the stimulation of de novo lipogenesis in both cell models, resulting in the induction of lipid accumulation. In contrast with a PPARgamma agonist (BRL49653), T0901317 enhanced only slightly the expression of PPARgamma dependent genes (PPARgamma, aP2 and adiponectin) in Chub-S7 cells and failed to change their expression in human SVF cells. These results show that LXR stimulated preferentially triglyceride accumulation in human preadipocytes via the induction of de novo lipogenesis, rather than activating the differentiation process through PPARgamma activation.
Mots-clé
Adipocytes/cytology/*metabolism Biological Markers Cell Differentiation Cells, Cultured DNA-Binding Proteins/*metabolism/pharmacology Gene Expression Regulation Humans *Lipogenesis PPAR gamma/metabolism Receptors, Cytoplasmic and Nuclear/*metabolism Stem Cells/*metabolism Stromal Cells/metabolism Transcription Factors/metabolism Triglycerides/biosynthesis/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 17:23
Dernière modification de la notice
20/08/2019 17:04
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