SPOT14-positive neural stem/progenitor cells in the hippocampus respond dynamically to neurogenic regulators.

Détails

Ressource 1Télécharger: Knobloch et al. 2014 Stem Cell Reports.pdf (8223.85 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_D9353C50A828
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
SPOT14-positive neural stem/progenitor cells in the hippocampus respond dynamically to neurogenic regulators.
Périodique
Stem cell reports
Auteur(s)
Knobloch M., von Schoultz C., Zurkirchen L., Braun S.M., Vidmar M., Jessberger S.
ISSN
2213-6711 (Electronic)
ISSN-L
2213-6711
Statut éditorial
Publié
Date de publication
11/11/2014
Peer-reviewed
Oui
Volume
3
Numéro
5
Pages
735-742
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Proliferation of neural stem/progenitor cells (NSPCs) in the adult brain is tightly controlled to prevent exhaustion and to ensure proper neurogenesis. Several extrinsic stimuli affect NSPC regulation. However, the lack of unique markers led to controversial results regarding the in vivo behavior of NSPCs to different stimuli. We recently identified SPOT14, which controls NSPC proliferation through regulation of de novo lipogenesis, selectively in low-proliferating NSPCs. Whether SPOT14-expressing (SPOT14+) NSPCs react in vivo to neurogenic regulators is not known. We show that aging is accompanied by a marked disappearance of SPOT14+ NSPCs, whereas running, a positive neurogenic stimulus, increases proliferation of SPOT14+ NSPCs. Furthermore, transient depletion of highly proliferative cells recruits SPOT14+ NSPCs into the proliferative pool. Additionally, we have established endogenous SPOT14 protein staining, reflecting transgenic SPOT14-GFP expression. Thus, our data identify SPOT14 as a potent marker for adult NSPCs that react dynamically to positive and negative neurogenic regulators.
Mots-clé
Age Factors, Animals, Antineoplastic Agents, Alkylating/pharmacology, Biomarkers/metabolism, Cell Proliferation/drug effects, Dacarbazine/analogs & derivatives, Dacarbazine/pharmacology, Green Fluorescent Proteins/genetics, Green Fluorescent Proteins/metabolism, Hippocampus/cytology, Hippocampus/growth & development, Hippocampus/metabolism, Immunohistochemistry, Mice, Transgenic, Microscopy, Fluorescence, Neural Stem Cells/metabolism, Neurogenesis/drug effects, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/05/2018 9:28
Dernière modification de la notice
09/05/2019 2:00
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