Use of an autonomous parvovirus vector for selective transfer of a foreign gene into transformed human cells of different tissue origins and its expression therein.

Détails

ID Serval
serval:BIB_D89F1852401E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Use of an autonomous parvovirus vector for selective transfer of a foreign gene into transformed human cells of different tissue origins and its expression therein.
Périodique
Journal of Virology
Auteur(s)
Dupont F., Tenenbaum L., Guo L.P., Spegelaere P., Zeicher M., Rommelaere J.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
1994
Volume
68
Numéro
3
Pages
1397-1406
Langue
anglais
Résumé
In this work, we report the transduction of a chloramphenicol acetyltransferase (CAT) reporter gene into a variety of normal and transformed human cells of various tissue origins. The vector used was MVM/P38cat, a recombinant of the prototype strain of the autonomous parvovirus minute virus of mice (MVMp). The CAT gene was inserted into the capsid-encoding region of the infectious molecular clone of MVMp genome, under the control of the MVM P38 promoter. When used to transfect permissive cells, the MVM/P38cat DNA was efficiently replicated and expressed the foreign CAT gene at high levels. By cotransfecting with a helper plasmid expressing the capsid proteins, it was possible to produce mixed virus stocks containing MVM/P38cat infectious particles and variable amounts of recombinant MVM. MVM/P38cat viral particles were successfully used to transfer the CAT gene and to express it in a variety of human cells. Both viral DNA replication and P38-driven CAT expression were achieved in fibroblasts, epithelial cells, T lymphocytes, and macrophages in a transformation-dependent way, but with an efficiency depending on the cell type. In transformed B lymphocytes, however, the vector was not replicated, nor did it express the CAT gene.
Mots-clé
Animals, Cell Line, Transformed, Chloramphenicol O-Acetyltransferase/biosynthesis, Chloramphenicol O-Acetyltransferase/genetics, DNA Replication, Epithelial Cells, Epithelium/microbiology, Fibroblasts/cytology, Fibroblasts/microbiology, Gene Transfer Techniques, Genetic Vectors/genetics, Humans, Macrophages/cytology, Macrophages/microbiology, Minute virus of mice/genetics, Minute virus of mice/growth & development, T-Lymphocytes/cytology, T-Lymphocytes/microbiology, Transduction, Genetic, Transfection, Virus Replication
Pubmed
Web of science
Création de la notice
01/04/2011 11:15
Dernière modification de la notice
03/03/2018 21:51
Données d'usage