Use of an autonomous parvovirus vector for selective transfer of a foreign gene into transformed human cells of different tissue origins and its expression therein.
Détails
ID Serval
serval:BIB_D89F1852401E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Use of an autonomous parvovirus vector for selective transfer of a foreign gene into transformed human cells of different tissue origins and its expression therein.
Périodique
Journal of Virology
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
1994
Volume
68
Numéro
3
Pages
1397-1406
Langue
anglais
Résumé
In this work, we report the transduction of a chloramphenicol acetyltransferase (CAT) reporter gene into a variety of normal and transformed human cells of various tissue origins. The vector used was MVM/P38cat, a recombinant of the prototype strain of the autonomous parvovirus minute virus of mice (MVMp). The CAT gene was inserted into the capsid-encoding region of the infectious molecular clone of MVMp genome, under the control of the MVM P38 promoter. When used to transfect permissive cells, the MVM/P38cat DNA was efficiently replicated and expressed the foreign CAT gene at high levels. By cotransfecting with a helper plasmid expressing the capsid proteins, it was possible to produce mixed virus stocks containing MVM/P38cat infectious particles and variable amounts of recombinant MVM. MVM/P38cat viral particles were successfully used to transfer the CAT gene and to express it in a variety of human cells. Both viral DNA replication and P38-driven CAT expression were achieved in fibroblasts, epithelial cells, T lymphocytes, and macrophages in a transformation-dependent way, but with an efficiency depending on the cell type. In transformed B lymphocytes, however, the vector was not replicated, nor did it express the CAT gene.
Mots-clé
Animals, Cell Line, Transformed, Chloramphenicol O-Acetyltransferase/biosynthesis, Chloramphenicol O-Acetyltransferase/genetics, DNA Replication, Epithelial Cells, Epithelium/microbiology, Fibroblasts/cytology, Fibroblasts/microbiology, Gene Transfer Techniques, Genetic Vectors/genetics, Humans, Macrophages/cytology, Macrophages/microbiology, Minute virus of mice/genetics, Minute virus of mice/growth & development, T-Lymphocytes/cytology, T-Lymphocytes/microbiology, Transduction, Genetic, Transfection, Virus Replication
Pubmed
Web of science
Création de la notice
01/04/2011 11:15
Dernière modification de la notice
20/08/2019 16:58