Use of an autonomous parvovirus vector for selective transfer of a foreign gene into transformed human cells of different tissue origins and its expression therein.

Details

Serval ID
serval:BIB_D89F1852401E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Use of an autonomous parvovirus vector for selective transfer of a foreign gene into transformed human cells of different tissue origins and its expression therein.
Journal
Journal of Virology
Author(s)
Dupont F., Tenenbaum L., Guo L.P., Spegelaere P., Zeicher M., Rommelaere J.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Publication state
Published
Issued date
1994
Volume
68
Number
3
Pages
1397-1406
Language
english
Abstract
In this work, we report the transduction of a chloramphenicol acetyltransferase (CAT) reporter gene into a variety of normal and transformed human cells of various tissue origins. The vector used was MVM/P38cat, a recombinant of the prototype strain of the autonomous parvovirus minute virus of mice (MVMp). The CAT gene was inserted into the capsid-encoding region of the infectious molecular clone of MVMp genome, under the control of the MVM P38 promoter. When used to transfect permissive cells, the MVM/P38cat DNA was efficiently replicated and expressed the foreign CAT gene at high levels. By cotransfecting with a helper plasmid expressing the capsid proteins, it was possible to produce mixed virus stocks containing MVM/P38cat infectious particles and variable amounts of recombinant MVM. MVM/P38cat viral particles were successfully used to transfer the CAT gene and to express it in a variety of human cells. Both viral DNA replication and P38-driven CAT expression were achieved in fibroblasts, epithelial cells, T lymphocytes, and macrophages in a transformation-dependent way, but with an efficiency depending on the cell type. In transformed B lymphocytes, however, the vector was not replicated, nor did it express the CAT gene.
Keywords
Animals, Cell Line, Transformed, Chloramphenicol O-Acetyltransferase/biosynthesis, Chloramphenicol O-Acetyltransferase/genetics, DNA Replication, Epithelial Cells, Epithelium/microbiology, Fibroblasts/cytology, Fibroblasts/microbiology, Gene Transfer Techniques, Genetic Vectors/genetics, Humans, Macrophages/cytology, Macrophages/microbiology, Minute virus of mice/genetics, Minute virus of mice/growth & development, T-Lymphocytes/cytology, T-Lymphocytes/microbiology, Transduction, Genetic, Transfection, Virus Replication
Pubmed
Web of science
Create date
01/04/2011 11:15
Last modification date
20/08/2019 16:58
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