Accuracy profile validation of a new analytical method for propane measurement using headspace-gas chromatography-mass spectrometry

Détails

Ressource 1Télécharger: serval:BIB_D6A683B15430.P001 (259.16 [Ko])
Etat: Public
Version: de l'auteur
Licence: Non spécifiée
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ID Serval
serval:BIB_D6A683B15430
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Accuracy profile validation of a new analytical method for propane measurement using headspace-gas chromatography-mass spectrometry
Périodique
Journal of Analytical Toxicology
Auteur(s)
Smith F., Augsburger M., Varlet V.
ISSN
1945-2403 (Electronic)
ISSN-L
0146-4760
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
38
Numéro
2
Pages
73-79
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Propane can be responsible for several types of lethal intoxication and explosions. Quantifying it would be very helpful to determine in some cases the cause of death. Some gas chromatography-mass spectrometry (GC-MS) methods of propane measurements do already exist. The main drawback of these GC-MS methods described in the literature is the absence of a specific propane internal standard necessary for accurate quantitative analysis. The main outcome of the following study was to provide an innovative Headspace-GC-MS method (HS-GC-MS) applicable to the routine determination of propane concentration in forensic toxicology laboratories. To date, no stable isotope of propane is commercially available. The development of an in situ generation of standards is thus presented. An internal-labeled standard gas (C3DH7) is generated in situ by the stoichiometric formation of propane by the reaction of deuterated water (D2O) with Grignard reagent propylmagnesium chloride (C3H7MgCl). The method aims to use this internal standard to quantify propane concentrations and, therefore, to obtain precise measurements. Consequently, a complete validation with an accuracy profile according to two different guidelines, the French Society of Pharmaceutical Sciences and Techniques (SFSTP) and the Gesellschaft für toxikologische und Forensische Chemie (GTFCh), is presented.
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/12/2013 15:18
Dernière modification de la notice
25/09/2019 6:10
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