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Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor.
Publication types: Journal Article. - Old month value: Mar 28
The aim of the present work was to study the effect of angiotensin II (Ang II) on catecholamines and neuropeptide Y (NPY) release in primary cultures of human adrenal chromaffin cells. Ang II stimulates norepinephrine (NE), epinephrine (EP) and NPY release from perifused chromaffin cells by 3-, 2- and 12-fold, respectively. The NPY release is more sustained than that of catecholamines. We found that the receptor-AT(2) agonist, T(2)-(Ang II 4-8)(2) has no effect on NE, EP and NPY release from chromaffin cells. We further showed that Ang II increases intracellular Ca(2+) concentration ([Ca(2+)](i)). The selective AT(1)-receptor antagonist Candesartan blocked [Ca(2+)](i) increase by Ang II, while T(2)-(Ang II 4-8)(2) was ineffective. These findings demonstrate that AT(1) stimulation induces catecholamine secretion from human adrenal chromaffin cells probably by raising cytosolic calcium.
Adrenal Glands/cytology, Angiotensin II/metabolism, Angiotensin II/pharmacology, Angiotensin II Type 1 Receptor Blockers, Calcium/chemistry, Calcium/metabolism, Cells, Cultured, Chromaffin Cells/drug effects, Chromaffin Cells/metabolism, Epinephrine/secretion, Humans, Neuropeptide Y/secretion, Norepinephrine/secretion, Receptor, Angiotensin, Type 1/agonists, Receptor, Angiotensin, Type 1/metabolism, Signal Transduction
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