Article: article from journal or magazin.
Molecular identification of an endemic Alpine mammal, Apodemus alpicola, using a PCR-based RFLP method
Revue Suisse de Zoologie
The ability of a PCR-based restriction fragment length polymorphism (RFLP) analysis of the cytochrome b (mtDNA) to distinguish Apodemus alpicola from two other Apodemus species was investigated. The partial sequencing of the cytochrome b allowed the identification of one enzyme as being potentially diagnostic. This was supported by an analysis of 131 specimens previously identified using morphometric and/or allozymic data, indicating that the PCR-based RFLP method provides a rapid and reliable tool for distinguishing A. alpicola from its two co-occurring congenerics. The method is applicable to samples taken in the field for ecological studies, and could easily be adapted to the identification of museum samples.
apodemus mtdna pcr rflp identification mitochondrial-DNA sylvaticus flavicollis populations divergence rodentia
Web of science
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