Molecular identification of an endemic Alpine mammal, Apodemus alpicola, using a PCR-based RFLP method

Détails

ID Serval
serval:BIB_D17DA8152E4E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Molecular identification of an endemic Alpine mammal, Apodemus alpicola, using a PCR-based RFLP method
Périodique
Revue Suisse de Zoologie
Auteur(s)
Reutter B. A., Petit E., Vogel P.
ISSN
0035-418X
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
109
Numéro
1
Pages
9-16
Langue
anglais
Résumé
The ability of a PCR-based restriction fragment length polymorphism (RFLP) analysis of the cytochrome b (mtDNA) to distinguish Apodemus alpicola from two other Apodemus species was investigated. The partial sequencing of the cytochrome b allowed the identification of one enzyme as being potentially diagnostic. This was supported by an analysis of 131 specimens previously identified using morphometric and/or allozymic data, indicating that the PCR-based RFLP method provides a rapid and reliable tool for distinguishing A. alpicola from its two co-occurring congenerics. The method is applicable to samples taken in the field for ecological studies, and could easily be adapted to the identification of museum samples.
Mots-clé
apodemus mtdna pcr rflp identification mitochondrial-DNA sylvaticus flavicollis populations divergence rodentia
Web of science
Création de la notice
24/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:51
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