Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN.

Détails

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Etat: Serval
Version: Final published version
ID Serval
serval:BIB_C88C3A4AFB89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN.
Périodique
Journal of Cell Science
Auteur(s)
Wachowicz P., Chasapi A., Krapp A., Cano Del Rosario E., Schmitter D., Sage D., Unser M., Xenarios I., Rougemont J., Simanis V.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Statut éditorial
Publié
Date de publication
2015
Volume
128
Numéro
4
Pages
741-754
Langue
anglais
Résumé
The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis to study SIN proteins in large numbers of wild-type and mutant cells. Our principal conclusions are: first, that the association of Cdc7p with the SPBs in early mitosis is frequently asymmetric, with a bias in favour of the new SPB; second, that the early association of Cdc7p-GFP to the SPB depends on Plo1p but not Spg1p, and is unaffected by mutations that influence its asymmetry in anaphase; third, that Cdc7p asymmetry in anaphase B is delayed by Pom1p and by activation of the spindle assembly checkpoint, and is promoted by Rad24p; and fourth, that the length of the spindle, expressed as a fraction of the length of the cell, at which Cdc7p becomes asymmetric is similar in cells dividing at different sizes. These data reveal that multiple regulatory mechanisms control the SIN in mitosis and lead us to propose a two-state model to describe the SIN.
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/02/2015 18:41
Dernière modification de la notice
09/05/2019 1:09
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