Cx47 fine-tunes the handling of serum lipids but is dispensable for lymphatic vascular function.

Details

Ressource 1Download: 28732089_BIB_C8459DCFE56A.pdf (3430.85 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_C8459DCFE56A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cx47 fine-tunes the handling of serum lipids but is dispensable for lymphatic vascular function.
Journal
PloS one
Author(s)
Meens M.J., Kutkut I., Rochemont V., Dubrot J., Kaladji F.R., Sabine A., Lyons O., Hendrikx S., Bernier-Latmani J., Kiefer F., Smith A., Hugues S., Petrova T.V., Kwak B.R.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
12
Number
7
Pages
e0181476
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Mutations in the gap junction protein connexin47 (Cx47) are associated with lymphedema. However, the role of Cx47 in lymphatic pathophysiology is unknown. We demonstrate that Cx47 is expressed in lymphatic endothelial cells by whole-mount immunostaining and qPCR. To determine if Cx47 plays a role in lymphatic vessel function we analysed Cx47-/- mice. Cx47-deficiency did not affect lymphatic contractility (contractile amplitude or frequency) or lymphatic morphology (vessel diameter or number of valves). Interstitial fluid drainage or dendritic cell migration through lymphatic vessels was also not affected by Cx47-deficiency. Cx47 is dispensable for long-chain fatty acid absorption from the gut but rather promotes serum lipid handling as prolonged elevated triglyceride levels were observed in Cx47-deficient mice after oral lipid tolerance tests. When crossed with Apolipoprotein E-deficient (Apoe-/-) mice, LDL-cholesterol was decreased in young Cx47-/-Apoe-/- adults as compared to Apoe-/- mice, which was inverted later in life. Finally, advanced atherosclerotic plaques in thoracic-abdominal aortas of 15 months-old mice tended to be larger in Cx47-/-Apoe-/- mice. These plaques contained fewer macrophages but similar amounts of T lymphocytes, collagen and lipids than plaques of Apoe-/- mice. In conclusion, Cx47 is expressed in lymphatic endothelium and seems modestly implicated in multiple aspects of lymphatic pathophysiology.
Pubmed
Web of science
Open Access
Yes
Create date
28/08/2017 10:26
Last modification date
30/04/2021 6:14
Usage data