Cx47 fine-tunes the handling of serum lipids but is dispensable for lymphatic vascular function.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_C8459DCFE56A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cx47 fine-tunes the handling of serum lipids but is dispensable for lymphatic vascular function.
Périodique
PloS one
Auteur(s)
Meens M.J., Kutkut I., Rochemont V., Dubrot J., Kaladji F.R., Sabine A., Lyons O., Hendrikx S., Bernier-Latmani J., Kiefer F., Smith A., Hugues S., Petrova T.V., Kwak B.R.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
12
Numéro
7
Pages
e0181476
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Mutations in the gap junction protein connexin47 (Cx47) are associated with lymphedema. However, the role of Cx47 in lymphatic pathophysiology is unknown. We demonstrate that Cx47 is expressed in lymphatic endothelial cells by whole-mount immunostaining and qPCR. To determine if Cx47 plays a role in lymphatic vessel function we analysed Cx47-/- mice. Cx47-deficiency did not affect lymphatic contractility (contractile amplitude or frequency) or lymphatic morphology (vessel diameter or number of valves). Interstitial fluid drainage or dendritic cell migration through lymphatic vessels was also not affected by Cx47-deficiency. Cx47 is dispensable for long-chain fatty acid absorption from the gut but rather promotes serum lipid handling as prolonged elevated triglyceride levels were observed in Cx47-deficient mice after oral lipid tolerance tests. When crossed with Apolipoprotein E-deficient (Apoe-/-) mice, LDL-cholesterol was decreased in young Cx47-/-Apoe-/- adults as compared to Apoe-/- mice, which was inverted later in life. Finally, advanced atherosclerotic plaques in thoracic-abdominal aortas of 15 months-old mice tended to be larger in Cx47-/-Apoe-/- mice. These plaques contained fewer macrophages but similar amounts of T lymphocytes, collagen and lipids than plaques of Apoe-/- mice. In conclusion, Cx47 is expressed in lymphatic endothelium and seems modestly implicated in multiple aspects of lymphatic pathophysiology.
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/08/2017 11:26
Dernière modification de la notice
30/04/2021 7:14
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