Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins.
Details
Serval ID
serval:BIB_C4EBDB424993
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins.
Journal
Journal of Infectious Diseases
Working group(s)
International Collaboration on Endocarditis-Microbiology Investigators
Contributor(s)
Athan E., Harris O., Korman TM., Kotsanas D., Jones P., Reinbott P., Ryan S., Fortes CQ., Garcia P., Jones SB., Barsic B., Bukovski S., Selton-Suty C., Aissa N., Doco-Lecompte T., Delahaye F., Vandenesch F., Tattevin P., Hoen B., Plesiat P., Giamarellou H., Giannitsioti E., Durante-Mangoni E., Françoise Tripodi M., Utili R., Casillo R., Cuccurullo S., Donnio PY., Raglio A., Suter F., Kanafani Z., Kanj SS., Morris A., Murdoch DR., Premru MM., Lejko-Zupanc T., Almela M., Armero Y., Azqueta M., Castan X., Cervera C., De Lazzari E., Rio Ad., Falces C., Garcia-de-la-Maria C., Gatell JM., Heras M., Marco F., Mestres CA., Miró JM., Moreno A., Ninot S., Paré C., Pericas J., Ramirez J., Sitges M., Bouza E., Rodríguez-Créixems M., Ramallo V., Bradley S., Wray D., Steed L., Cantey R., Peterson G., Stancoven A., Woods C., Corey GR., Reller LB., Fowler VG.<Suffix>Jr</Suffix> , Chu VH., Baloch K., Chu VH., Corey GR., Dixon CC., Fowler VG.<Suffix>Jr</Suffix> , Harding T., Pappas P., Park LP., Redick T., Jones-Richmond M., Stafford J., Anstrom K., Athan E., Bayer AS., Cabell CH., Chu VH., Corey GR., Fowler VG.<Suffix>Jr</Suffix> , Hoen B., Karchmer AW., Miró JM., Murdoch DR., Sexton DJ., Wang A., Bayer AS., Cabell CH., Chu V., Corey GR., Durack DT., Eykyn S., Fowler VG.<Suffix>Jr</Suffix> , Hoen B., Miró JM., Moreillon P., Olaison L., Raoult D., Rubinstein E., Sexton D.
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Publication state
Published
Issued date
2011
Volume
204
Number
5
Pages
704-713
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
BACKGROUND: Using multinational collections of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from infective endocarditis (IE) and soft tissue infections (STIs), we sought to (1) validate the finding that S. aureus in clonal complex (CC) 30 is associated with hematogenous complications and (2) test the hypothesis that specific genetic characteristics in S. aureus are associated with infection severity.
METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes.
RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5% vs 6.2%; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P < .0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P ≤ .005 for all). CC30 isolates were more likely to contain cna, tst, sea, see, seg, and chp (P < .05 for all).
CONCLUSIONS: MSSA IE isolates were significantly more likely to be CC30 and to possess a distinct repertoire of virulence genes than MSSA STI isolates from the same region. The genetic basis of this association requires further study.
METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes.
RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5% vs 6.2%; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P < .0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P ≤ .005 for all). CC30 isolates were more likely to contain cna, tst, sea, see, seg, and chp (P < .05 for all).
CONCLUSIONS: MSSA IE isolates were significantly more likely to be CC30 and to possess a distinct repertoire of virulence genes than MSSA STI isolates from the same region. The genetic basis of this association requires further study.
Keywords
Adhesins, Bacterial/genetics, Adult, Aged, Australia, Bacterial Typing Techniques, DNA, Bacterial/genetics, Endocarditis, Bacterial/genetics, Enterotoxins/genetics, Europe, Female, Genotype, Humans, Male, Methicillin Resistance, Middle Aged, Middle East, Multilocus Sequence Typing, New Zealand, North America, Severity of Illness Index, Soft Tissue Infections/genetics, Staphylococcal Infections/genetics, Staphylococcus aureus/genetics, Staphylococcus aureus/pathogenicity, Virulence Factors/genetics
Pubmed
Web of science
Create date
06/12/2011 16:41
Last modification date
20/08/2019 16:40
Usage data
