Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins.
Détails
ID Serval
serval:BIB_C4EBDB424993
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Methicillin-susceptible Staphylococcus aureus endocarditis isolates are associated with clonal complex 30 genotype and a distinct repertoire of enterotoxins and adhesins.
Périodique
Journal of Infectious Diseases
Collaborateur⸱rice⸱s
International Collaboration on Endocarditis-Microbiology Investigators
Contributeur⸱rice⸱s
Athan E., Harris O., Korman TM., Kotsanas D., Jones P., Reinbott P., Ryan S., Fortes CQ., Garcia P., Jones SB., Barsic B., Bukovski S., Selton-Suty C., Aissa N., Doco-Lecompte T., Delahaye F., Vandenesch F., Tattevin P., Hoen B., Plesiat P., Giamarellou H., Giannitsioti E., Durante-Mangoni E., Françoise Tripodi M., Utili R., Casillo R., Cuccurullo S., Donnio PY., Raglio A., Suter F., Kanafani Z., Kanj SS., Morris A., Murdoch DR., Premru MM., Lejko-Zupanc T., Almela M., Armero Y., Azqueta M., Castan X., Cervera C., De Lazzari E., Rio Ad., Falces C., Garcia-de-la-Maria C., Gatell JM., Heras M., Marco F., Mestres CA., Miró JM., Moreno A., Ninot S., Paré C., Pericas J., Ramirez J., Sitges M., Bouza E., Rodríguez-Créixems M., Ramallo V., Bradley S., Wray D., Steed L., Cantey R., Peterson G., Stancoven A., Woods C., Corey GR., Reller LB., Fowler VG.<Suffix>Jr</Suffix> , Chu VH., Baloch K., Chu VH., Corey GR., Dixon CC., Fowler VG.<Suffix>Jr</Suffix> , Harding T., Pappas P., Park LP., Redick T., Jones-Richmond M., Stafford J., Anstrom K., Athan E., Bayer AS., Cabell CH., Chu VH., Corey GR., Fowler VG.<Suffix>Jr</Suffix> , Hoen B., Karchmer AW., Miró JM., Murdoch DR., Sexton DJ., Wang A., Bayer AS., Cabell CH., Chu V., Corey GR., Durack DT., Eykyn S., Fowler VG.<Suffix>Jr</Suffix> , Hoen B., Miró JM., Moreillon P., Olaison L., Raoult D., Rubinstein E., Sexton D.
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Statut éditorial
Publié
Date de publication
2011
Volume
204
Numéro
5
Pages
704-713
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
BACKGROUND: Using multinational collections of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from infective endocarditis (IE) and soft tissue infections (STIs), we sought to (1) validate the finding that S. aureus in clonal complex (CC) 30 is associated with hematogenous complications and (2) test the hypothesis that specific genetic characteristics in S. aureus are associated with infection severity.
METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes.
RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5% vs 6.2%; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P < .0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P ≤ .005 for all). CC30 isolates were more likely to contain cna, tst, sea, see, seg, and chp (P < .05 for all).
CONCLUSIONS: MSSA IE isolates were significantly more likely to be CC30 and to possess a distinct repertoire of virulence genes than MSSA STI isolates from the same region. The genetic basis of this association requires further study.
METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes.
RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5% vs 6.2%; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P < .0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P ≤ .005 for all). CC30 isolates were more likely to contain cna, tst, sea, see, seg, and chp (P < .05 for all).
CONCLUSIONS: MSSA IE isolates were significantly more likely to be CC30 and to possess a distinct repertoire of virulence genes than MSSA STI isolates from the same region. The genetic basis of this association requires further study.
Mots-clé
Adhesins, Bacterial/genetics, Adult, Aged, Australia, Bacterial Typing Techniques, DNA, Bacterial/genetics, Endocarditis, Bacterial/genetics, Enterotoxins/genetics, Europe, Female, Genotype, Humans, Male, Methicillin Resistance, Middle Aged, Middle East, Multilocus Sequence Typing, New Zealand, North America, Severity of Illness Index, Soft Tissue Infections/genetics, Staphylococcal Infections/genetics, Staphylococcus aureus/genetics, Staphylococcus aureus/pathogenicity, Virulence Factors/genetics
Pubmed
Web of science
Création de la notice
06/12/2011 16:41
Dernière modification de la notice
20/08/2019 16:40
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