In vitro evaluation of gel-encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair.

Details

Serval ID
serval:BIB_C4E2794315E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro evaluation of gel-encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair.
Journal
Journal of tissue engineering and regenerative medicine
Author(s)
de Luca A.C., Fonta C.M., Raffoul W., di Summa P.G. (co-last), Lacour S.P.
ISSN
1932-7005 (Electronic)
ISSN-L
1932-6254
Publication state
Published
Issued date
03/2018
Peer-reviewed
Oui
Volume
12
Number
3
Pages
676-686
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Adipose-derived stem cells (ASC) are becoming one of the most exploited cells in peripheral nerve repair. They are fast-growing and able to protect neurons from apoptosis; they can reduce post-injury latency and the risk of muscle atrophy. This study evaluates laminin-loaded fibrin gel as an ASC-carrying scaffold for nerve repair. In vitro, ASC retained their proliferative activity but showed significant increase in proliferation rate when encapsulated in gels with low laminin concentrations (i.e., 1 μg/mL). We observed a linear decrease of ASC proliferation rate with increasing laminin concentration from 1 to 100 μg/mL. We next examined the effect of the ASC-carrying fibrin gels on in vitro dorsal root ganglia (DRG) neurite extension, then in vivo sciatic nerve regeneration in adult rats. The ASC-carrying gel was embedded in 15-mm-long, 1.5-mm-diameter polydimethylsiloxane regenerative conduits for in vivo evaluation. At 8-week post implantation, robust regeneration was observed across the long gap. Taken together, these results suggest ASC-carrying gels are a potential path to improve the efficacy of nerve regeneration through artificial guidance conduits and electrode nerve interfaces.
Keywords
Adhesiveness, Adipose Tissue/cytology, Animals, Cell Proliferation/drug effects, Cell Survival/drug effects, Cells, Immobilized/cytology, Coculture Techniques, Dimethylpolysiloxanes/chemistry, Fibrin/pharmacology, Ganglia, Spinal/drug effects, Ganglia, Spinal/metabolism, Gels/chemistry, Laminin/pharmacology, Male, Mesenchymal Stem Cells/cytology, Mesenchymal Stem Cells/drug effects, Mesenchymal Stem Cells/metabolism, Nerve Regeneration, Peripheral Nerves/pathology, Rats, Sprague-Dawley, Sciatic Nerve/drug effects, adipose stem cells, cell transplantation, fibrin, laminin, nerve regeneration
Pubmed
Web of science
Create date
06/06/2017 18:28
Last modification date
17/06/2023 5:53
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