In vitro evaluation of gel-encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair.

Détails

ID Serval
serval:BIB_C4E2794315E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro evaluation of gel-encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair.
Périodique
Journal of tissue engineering and regenerative medicine
Auteur(s)
de Luca A.C., Fonta C.M., Raffoul W., di Summa P.G., Lacour S.P.
ISSN
1932-7005 (Electronic)
ISSN-L
1932-6254
Statut éditorial
Publié
Date de publication
03/2018
Peer-reviewed
Oui
Volume
12
Numéro
3
Pages
676-686
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Adipose-derived stem cells (ASC) are becoming one of the most exploited cells in peripheral nerve repair. They are fast-growing and able to protect neurons from apoptosis; they can reduce post-injury latency and the risk of muscle atrophy. This study evaluates laminin-loaded fibrin gel as an ASC-carrying scaffold for nerve repair. In vitro, ASC retained their proliferative activity but showed significant increase in proliferation rate when encapsulated in gels with low laminin concentrations (i.e., 1 μg/mL). We observed a linear decrease of ASC proliferation rate with increasing laminin concentration from 1 to 100 μg/mL. We next examined the effect of the ASC-carrying fibrin gels on in vitro dorsal root ganglia (DRG) neurite extension, then in vivo sciatic nerve regeneration in adult rats. The ASC-carrying gel was embedded in 15-mm-long, 1.5-mm-diameter polydimethylsiloxane regenerative conduits for in vivo evaluation. At 8-week post implantation, robust regeneration was observed across the long gap. Taken together, these results suggest ASC-carrying gels are a potential path to improve the efficacy of nerve regeneration through artificial guidance conduits and electrode nerve interfaces.
Mots-clé
Adhesiveness, Adipose Tissue/cytology, Animals, Cell Proliferation/drug effects, Cell Survival/drug effects, Cells, Immobilized/cytology, Coculture Techniques, Dimethylpolysiloxanes/chemistry, Fibrin/pharmacology, Ganglia, Spinal/drug effects, Ganglia, Spinal/metabolism, Gels/chemistry, Laminin/pharmacology, Male, Mesenchymal Stem Cells/cytology, Mesenchymal Stem Cells/drug effects, Mesenchymal Stem Cells/metabolism, Nerve Regeneration, Peripheral Nerves/pathology, Rats, Sprague-Dawley, Sciatic Nerve/drug effects, adipose stem cells, cell transplantation, fibrin, laminin, nerve regeneration
Pubmed
Web of science
Création de la notice
06/06/2017 18:28
Dernière modification de la notice
22/10/2019 5:11
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