Expression of FGF-2 in neural progenitor cells enhances their potential for cellular brain repair in the rodent cortex.
Details
Serval ID
serval:BIB_C3EB0972D9E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of FGF-2 in neural progenitor cells enhances their potential for cellular brain repair in the rodent cortex.
Journal
Brain
ISSN
1460-2156 (Electronic)
ISSN-L
0006-8950
Publication state
Published
Issued date
11/2007
Peer-reviewed
Oui
Volume
130
Number
Pt 11
Pages
2962-2976
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Strategies to enhance the capacity of grafted stem/progenitors cells to generate multipotential, proliferative and migrating pools of cells in the postnatal brain could be crucial for structural repair after brain damage. We investigated whether the over-expression of basic fibroblast growth factor 2 (FGF-2) in neural progenitor cells (NPCs) could provide a robust source of migrating NPCs for tissue repair in the rat cerebral cortex. Using live imaging we provide direct evidence that FGF-2 over-expression significantly enhances the migratory capacity of grafted NPCs in complex 3D structures, such as cortical slices. Furthermore, we show that the migratory as well as proliferative properties of FGF-2 over-expressing NPCs are maintained after in vivo transplantation. Importantly, after transplantation into a neonatal ischaemic cortex, FGF-2 over-expressing NPCs efficiently invade the injured cortex and generate an increased pool of immature neurons available for brain repair. Differentiation of progenitor cells into immature neurons was correlated with a gradual down-regulation of the FGF-2 transgene. These results reveal an important role for FGF-2 in regulating NPCs functions when interacting with the host tissue and offer a potential strategy to generate a robust source of migrating and immature progenitors for repairing a neonatal ischaemic cortex.
Keywords
Animals, Animals, Newborn, Cell Movement, Cell Proliferation, Cerebral Cortex/chemistry, Cerebral Cortex/injuries, Cerebral Cortex/pathology, Fibroblast Growth Factor 2/analysis, Fibroblast Growth Factor 2/genetics, Fibroblast Growth Factor 2/metabolism, Gene Expression, Genetic Engineering, Genetic Vectors/administration & dosage, Genetic Vectors/genetics, HIV-1/genetics, Humans, Hypoxia-Ischemia, Brain/surgery, Immunohistochemistry, Microscopy, Fluorescence, Models, Animal, Rats, Rats, Sprague-Dawley, Stem Cell Transplantation/methods, Stem Cells/metabolism, Stem Cells/pathology, Transduction, Genetic/methods, Transgenes, Wound Healing
Pubmed
Web of science
Open Access
Yes
Create date
19/03/2021 15:44
Last modification date
20/03/2021 7:26