Digital image DNA cytometry: a useful tool for the evaluation of malignancy in biliary strictures.

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Version: Final published version
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Serval ID
serval:BIB_BDD7C80AC8D7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Digital image DNA cytometry: a useful tool for the evaluation of malignancy in biliary strictures.
Journal
Cellular oncology
Author(s)
Osterheld M.C., Andrejevic Blant S., Caron L., Braunschweig R., Dorta G., Bouzourene H., Mihaescu A.
ISSN
1570-5870
ISSN-L
1570-5870
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
27
Number
4
Pages
255-260
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cytologic evaluation of the biliary tract strictures is nowadays widely used for distinguishing between benign and malignant lesions but remains a challenge for some problematic cases. Digital Image cytometry (DNA-cytometry) helps cytopathologists to resolve some unclear situations.
We have analysed 41 specimens of bile duct brushings obtained from patients during ERCP (11 benign cases, 7 suspicious for malignancy cases and 23 malignant cases) by DNA-cytometry and correlated them with the histological biopsy counterpart.
All eleven cytological and histological benign cases were DNA-diploid and among 22 patients with malignant cytological and histological diagnosis 21 were DNA-aneuploid. One case considered malignant by the cytopathologist revealed DNA-aneuploid but malignancy could not be confirmed by histology. The analysis of the suspicious for malignancy cases revealed that all DNA-aneuploid cases were malignant and all DNA-diploid cases were benign referring to the follow-up of the patients. The comparison between cytology alone and cytology combined with DNA-cytometry related to the histological diagnosis (gold standard) resulted in a sensitivity of 100% and a specificity of 79% for cytology alone; a specificity of 94% and a sensitivity 92% for DNA-cytometry and a specificity of 93% and a sensitivity of 100% with combined analyses. The positive predictive value was 90% for cytology, 96% for DNA-cytometry and for both analyses. The negative predictive value showed 100% for cytology, 89% for DNA-cytometry and 100% for combined studies.
Despite the limited number of patients involved in the study, the results obtained indicate an increased of specificity and of positive predictive value using DNA-cytometry. These results confirm the pertinence of these method for challenging cases, in conjunction with other available diagnostic tools.
Keywords
Adult, Aged, Aged, 80 and over, Biliary Tract/cytology, Biliary Tract/metabolism, Biliary Tract/pathology, Biliary Tract Neoplasms/diagnosis, Biliary Tract Neoplasms/genetics, Biliary Tract Neoplasms/pathology, DNA, Neoplasm/genetics, Epithelial Cells/cytology, Epithelial Cells/metabolism, Epithelial Cells/pathology, Female, Humans, Image Cytometry, Male, Middle Aged
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:48
Last modification date
09/08/2024 14:53
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