Digital image DNA cytometry: a useful tool for the evaluation of malignancy in biliary strictures
Détails
ID Serval
serval:BIB_BDD7C80AC8D7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Digital image DNA cytometry: a useful tool for the evaluation of malignancy in biliary strictures
Périodique
Cellular Oncology
ISSN
1570-5870 (Print)
Statut éditorial
Publié
Date de publication
2005
Volume
27
Numéro
4
Pages
255-60
Notes
Journal Article
Résumé
BACKGROUND: Cytologic evaluation of the biliary tract strictures is nowadays widely used for distinguishing between benign and malignant lesions but remains a challenge for some problematic cases. Digital Image cytometry (DNA-cytometry) helps cytopathologists to resolve some unclear situations. METHODS: We have analysed 41 specimens of bile duct brushings obtained from patients during ERCP (11 benign cases, 7 suspicious for malignancy cases and 23 malignant cases) by DNA-cytometry and correlated them with the histological biopsy counterpart. RESULTS: All eleven cytological and histological benign cases were DNA-diploid and among 22 patients with malignant cytological and histological diagnosis 21 were DNA-aneuploid. One case considered malignant by the cytopathologist revealed DNA-aneuploid but malignancy could not be confirmed by histology. The analysis of the suspicious for malignancy cases revealed that all DNA-aneuploid cases were malignant and all DNA-diploid cases were benign referring to the follow-up of the patients. The comparison between cytology alone and cytology combined with DNA-cytometry related to the histological diagnosis (gold standard) resulted in a sensitivity of 100% and a specificity of 79% for cytology alone; a specificity of 94% and a sensitivity 92% for DNA-cytometry and a specificity of 93% and a sensitivity of 100% with combined analyses. The positive predictive value was 90% for cytology, 96% for DNA-cytometry and for both analyses. The negative predictive value showed 100% for cytology, 89% for DNA-cytometry and 100% for combined studies. CONCLUSIONS: Despite the limited number of patients involved in the study, the results obtained indicate an increased of specificity and of positive predictive value using DNA-cytometry. These results confirm the pertinence of these method for challenging cases, in conjunction with other available diagnostic tools.
Mots-clé
Adult
Aged
Aged, 80 and over
Biliary Tract/cytology/metabolism/*pathology
Biliary Tract Neoplasms/*diagnosis/*genetics/pathology
DNA, Neoplasm/genetics
Epithelial Cells/cytology/metabolism/pathology
Female
Humans
*Image Cytometry
Male
Middle Aged
Pubmed
Web of science
Création de la notice
25/01/2008 15:48
Dernière modification de la notice
20/08/2019 15:32