MHC class I expression dependent on bacterial infection and parental factors in whitefish embryos (Salmonidae).

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_BCE74E386F8B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MHC class I expression dependent on bacterial infection and parental factors in whitefish embryos (Salmonidae).
Périodique
Molecular Ecology
Auteur(s)
Clark E.S., Wilkins L.G., Wedekind C.
ISSN
1365-294X (Electronic)
ISSN-L
0962-1083
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
22
Numéro
20
Pages
5256-5269
Langue
anglais
Résumé
Ecological conditions can influence not only the expression of a phenotype, but also the heritability of a trait. As such, heritable variation for a trait needs to be studied across environments. We have investigated how pathogen challenge affects the expression of MHC genes in embryos of the lake whitefish Coregonus palaea. In order to experimentally separate paternal (i.e. genetic) from maternal and environmental effects, and determine whether and how stress affects the heritable variation for MHC expression, embryos were produced in full-factorial in vitro fertilizations, reared singly, and exposed at 208 degree days (late-eyed stage) to either one of two strains of Pseudomonas fluorescens that differ in their virulence characteristics (one increased mortality, while both delayed hatching time). Gene expression was assessed 48 h postinoculation, and virulence effects of the bacterial infection were monitored until hatching. We found no evidence of MHC class II expression at this stage of development. MHC class I expression was markedly down-regulated in reaction to both pseudomonads. While MHC expression could not be linked to embryo survival, the less the gene was expressed, the earlier the embryos hatched within each treatment group, possibly due to trade-offs between immune function and developmental rate or further factors that affect both hatching timing and MHC expression. We found significant additive genetic variance for MHC class I expression in some treatments. That is, changes in pathogen pressures could induce rapid evolution in MHC class I expression. However, we found no additive genetic variance in reaction norms in our study population.
Mots-clé
Coregonus, genetic variation, life history, plasticity, Pseudomonas fluorescens
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/07/2013 5:47
Dernière modification de la notice
20/08/2019 15:31
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