Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.
Details
Serval ID
serval:BIB_B80C2EE9D387
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.
Journal
Journal of Clinical Microbiology
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
52
Number
11
Pages
4033-4035
Language
english
Abstract
HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific for the other HPV genotypes during a 1-year prospective validation (n = 714 samples).
Keywords
DNA Primers/diagnostic use, DNA Primers/genetics, Female, Genotype, Humans, Molecular Diagnostic Techniques/methods, Oligonucleotide Probes/diagnostic use, Oligonucleotide Probes/genetics, Papillomaviridae/classification, Papillomaviridae/genetics, Papillomavirus Infections/diagnosis, Papillomavirus Infections/virology, Sensitivity and Specificity
Pubmed
Open Access
Yes
Create date
25/11/2015 15:56
Last modification date
20/08/2019 15:26
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