Article: article from journal or magazin.
Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.
Journal of Clinical Microbiology
HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific for the other HPV genotypes during a 1-year prospective validation (n = 714 samples).
DNA Primers/diagnostic use, DNA Primers/genetics, Female, Genotype, Humans, Molecular Diagnostic Techniques/methods, Oligonucleotide Probes/diagnostic use, Oligonucleotide Probes/genetics, Papillomaviridae/classification, Papillomaviridae/genetics, Papillomavirus Infections/diagnosis, Papillomavirus Infections/virology, Sensitivity and Specificity
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