Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.
Détails
ID Serval
serval:BIB_B80C2EE9D387
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.
Périodique
Journal of Clinical Microbiology
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
52
Numéro
11
Pages
4033-4035
Langue
anglais
Résumé
HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific for the other HPV genotypes during a 1-year prospective validation (n = 714 samples).
Mots-clé
DNA Primers/diagnostic use, DNA Primers/genetics, Female, Genotype, Humans, Molecular Diagnostic Techniques/methods, Oligonucleotide Probes/diagnostic use, Oligonucleotide Probes/genetics, Papillomaviridae/classification, Papillomaviridae/genetics, Papillomavirus Infections/diagnosis, Papillomavirus Infections/virology, Sensitivity and Specificity
Pubmed
Open Access
Oui
Création de la notice
25/11/2015 15:56
Dernière modification de la notice
20/08/2019 15:26
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