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Leishmania metacaspase: an arginine-specific peptidase.
Methods in Molecular Biology
The purpose of this chapter is to give insights into metacaspase of Leishmania protozoan parasites as arginine-specific cysteine peptidase. The physiological role of metacaspase in Leishmania is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (Δyca1) have been instrumental in studying the activity of Leishmania major metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.
Leishmania, Cysteine peptidase, Arginine-specific peptidase, Metacaspase, Enzymatic assay, Protease inhibitors
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