Leishmania metacaspase: an arginine-specific peptidase.

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Ressource 1Télécharger: BIB_B3F360C931E2.P001.pdf (697.98 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_B3F360C931E2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Leishmania metacaspase: an arginine-specific peptidase.
Périodique
Methods in Molecular Biology
Auteur(s)
Martin R., Gonzalez I., Fasel N.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
1133
Pages
189-202
Langue
anglais
Résumé
The purpose of this chapter is to give insights into metacaspase of Leishmania protozoan parasites as arginine-specific cysteine peptidase. The physiological role of metacaspase in Leishmania is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (Δyca1) have been instrumental in studying the activity of Leishmania major metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.
Mots-clé
Leishmania, Cysteine peptidase, Arginine-specific peptidase, Metacaspase, Enzymatic assay, Protease inhibitors
Pubmed
Web of science
Open Access
Oui
Création de la notice
26/06/2014 9:59
Dernière modification de la notice
20/08/2019 16:22
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