Article: article from journal or magazin.
Analysis of signal transducing mechanisms in CD3+ CD4- CD8- cells expressing the putative T cell receptor gamma gene product
Journal of Immunology
Journal Article --- Old month value: Dec 1
The signal transducing mechanisms, involved in the activation of CD3+ WT31- cells bearing the putative products of T cell receptor gamma genes, have been investigated. After stimulation with phytohemagglutinin or with monoclonal antibodies directed against CD2 or CD3 surface molecules, a rapid increase in free cytoplasmic Ca2+ concentration rise was detected in one representative CD3+ WT31- clone and in PEER cell line. Experiments performed in the presence of the Ca2+ chelator EGTA indicated that the free cytoplasmic Ca2+ concentration rise was consequent to an early release of Ca2+ from internal stores, followed by a sustained Ca2+ influx from the extracellular compartment. Moreover, increased levels of inositol-3-phosphate (the putative mobilizer of Ca2+ from the intracellular stores) were observed after stimulation, thus suggesting that activation of this cell subset occurs via the classical inositol-lipid metabolic pathway.
Antibodies, Monoclonal/immunology Antigens, Differentiation, T-Lymphocyte/*analysis Calcium/metabolism Humans Inositol Phosphates/metabolism *Lymphocyte Activation/drug effects Phytohemagglutinins/pharmacology Receptors, Antigen, T-Cell/*biosynthesis Receptors, Antigen, T-Cell, gamma-delta T-Lymphocytes/immunology/*physiology
Web of science
Last modification date