Analysis of signal transducing mechanisms in CD3+ CD4- CD8- cells expressing the putative T cell receptor gamma gene product

Détails

ID Serval
serval:BIB_A2356D572822
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Analysis of signal transducing mechanisms in CD3+ CD4- CD8- cells expressing the putative T cell receptor gamma gene product
Périodique
Journal of Immunology
Auteur⸱e⸱s
Pantaleo  G., Ferrini  S., Zocchi  M. R., Bottino  C., Biassoni  R., Moretta  L., Moretta  A.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
12/1987
Volume
139
Numéro
11
Pages
3580-4
Notes
Journal Article --- Old month value: Dec 1
Résumé
The signal transducing mechanisms, involved in the activation of CD3+ WT31- cells bearing the putative products of T cell receptor gamma genes, have been investigated. After stimulation with phytohemagglutinin or with monoclonal antibodies directed against CD2 or CD3 surface molecules, a rapid increase in free cytoplasmic Ca2+ concentration rise was detected in one representative CD3+ WT31- clone and in PEER cell line. Experiments performed in the presence of the Ca2+ chelator EGTA indicated that the free cytoplasmic Ca2+ concentration rise was consequent to an early release of Ca2+ from internal stores, followed by a sustained Ca2+ influx from the extracellular compartment. Moreover, increased levels of inositol-3-phosphate (the putative mobilizer of Ca2+ from the intracellular stores) were observed after stimulation, thus suggesting that activation of this cell subset occurs via the classical inositol-lipid metabolic pathway.
Mots-clé
Antibodies, Monoclonal/immunology Antigens, Differentiation, T-Lymphocyte/*analysis Calcium/metabolism Humans Inositol Phosphates/metabolism *Lymphocyte Activation/drug effects Phytohemagglutinins/pharmacology Receptors, Antigen, T-Cell/*biosynthesis Receptors, Antigen, T-Cell, gamma-delta T-Lymphocytes/immunology/*physiology
Pubmed
Web of science
Création de la notice
25/01/2008 15:13
Dernière modification de la notice
20/08/2019 15:08
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