Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.

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Version: Final published version
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Serval ID
serval:BIB_9FDB33F6CA9C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.
Journal
The Journal of cell biology
Author(s)
Muriel O., Michon L., Kukulski W., Martin S.G.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
04/10/2021
Peer-reviewed
Oui
Volume
220
Number
10
Pages
e202103142
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cell-cell fusion is central for sexual reproduction, and generally involves gametes of different shapes and sizes. In walled fission yeast Schizosaccharomyces pombe, the fusion of h+ and h- isogametes requires the fusion focus, an actin structure that concentrates glucanase-containing vesicles for cell wall digestion. Here, we present a quantitative correlative light and electron microscopy (CLEM) tomographic dataset of the fusion site, which reveals the fusion focus ultrastructure. Unexpectedly, gametes show marked asymmetries: a taut, convex plasma membrane of h- cells progressively protrudes into a more slack, wavy plasma membrane of h+ cells. Asymmetries are relaxed upon fusion, with observations of ramified fusion pores. h+ cells have a higher exo-/endocytosis ratio than h- cells, and local reduction in exocytosis strongly diminishes membrane waviness. Reciprocally, turgor pressure reduction specifically in h- cells impedes their protrusions into h+ cells and delays cell fusion. We hypothesize that asymmetric membrane conformations, due to differential turgor pressure and exocytosis/endocytosis ratios between mating types, favor cell-cell fusion.
Pubmed
Web of science
Create date
03/09/2021 16:18
Last modification date
01/03/2022 7:11
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