Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.
Détails
Télécharger: 2021_Muriel_JCB.pdf (3610.97 [Ko])
Etat: Public
Version: Final published version
Licence: Tous droits réservés
Etat: Public
Version: Final published version
Licence: Tous droits réservés
ID Serval
serval:BIB_9FDB33F6CA9C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.
Périodique
The Journal of cell biology
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Statut éditorial
Publié
Date de publication
04/10/2021
Peer-reviewed
Oui
Volume
220
Numéro
10
Pages
e202103142
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Cell-cell fusion is central for sexual reproduction, and generally involves gametes of different shapes and sizes. In walled fission yeast Schizosaccharomyces pombe, the fusion of h+ and h- isogametes requires the fusion focus, an actin structure that concentrates glucanase-containing vesicles for cell wall digestion. Here, we present a quantitative correlative light and electron microscopy (CLEM) tomographic dataset of the fusion site, which reveals the fusion focus ultrastructure. Unexpectedly, gametes show marked asymmetries: a taut, convex plasma membrane of h- cells progressively protrudes into a more slack, wavy plasma membrane of h+ cells. Asymmetries are relaxed upon fusion, with observations of ramified fusion pores. h+ cells have a higher exo-/endocytosis ratio than h- cells, and local reduction in exocytosis strongly diminishes membrane waviness. Reciprocally, turgor pressure reduction specifically in h- cells impedes their protrusions into h+ cells and delays cell fusion. We hypothesize that asymmetric membrane conformations, due to differential turgor pressure and exocytosis/endocytosis ratios between mating types, favor cell-cell fusion.
Pubmed
Web of science
Création de la notice
03/09/2021 17:18
Dernière modification de la notice
01/03/2022 8:11