The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes.

Détails

Ressource 1Télécharger: Yang_J Cell Sci-2013 - copie.pdf (1037.79 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_9E64C3251560
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes.
Périodique
Journal of Cell Science
Auteur(s)
Yang H.Y., Charles R.P., Hummler E., Baines D.L., Isseroff R.R.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Statut éditorial
Publié
Date de publication
2013
Volume
126
Numéro
Pt 9
Pages
1942-1951
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish. PDF type: Research article
Résumé
Cellular directional migration in an electric field (galvanotaxis) is one of the mechanisms guiding cell movement in embryogenesis and in skin epidermal repair. The epithelial sodium channel (ENaC), in addition to its function of regulating sodium transport in kidney, has recently been found to modulate cell locomotory speed. Here we tested whether ENaC has an additional function of mediating the directional migration of galvanotaxis in keratinocytes. Genetic depletion of ENaC completely blocks only galvanotaxis and does not decrease migration speed. Overexpression of ENaC is sufficient to drive galvanotaxis in otherwise unresponsive cells. Pharmacologic blockade or maintenance of the open state of ENaC also decreases or increases, respectively, galvanotaxis, suggesting that the channel open state is responsible for the response. Stable lamellipodial extensions formed at the cathodal sides of wild-type cells at the start of galvanotaxis; these were absent in the ENaC knockout keratinocytes, suggesting that ENaC mediates galvanotaxis by generating stable lamellipodia that steer cell migration. We provide evidence that ENaC is required for directional migration of keratinocytes in an electric field, supporting a role for ENaC in skin wound healing.
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/07/2013 19:50
Dernière modification de la notice
20/08/2019 15:04
Données d'usage