A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene Expression in Arabidopsis.

Details

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_9D02167F805B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene Expression in Arabidopsis.
Journal
Plant Physiology
Author(s)
Schürholz A.K., López-Salmerón V., Li Z., Forner J., Wenzl C., Gaillochet C., Augustin S., Barro A.V., Fuchs M., Gebert M., Lohmann J.U., Greb T., Wolf S.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
178
Number
1
Pages
40-53
Language
english
Abstract
Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell type-specific transactivation in Arabidopsis ( <i>Arabidopsis thaliana</i> ) based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic <i>pOp</i> promoter. Harnessing the flexibility of the GreenGate cloning system, we produced a comprehensive set of transgenic lines termed GR-LhG4 driver lines targeting most tissues in the Arabidopsis shoot and root with a strong focus on the indeterminate meristems. When we combined these transgenic lines with effectors under the control of the <i>pOp</i> promoter, we observed tight temporal and spatial control of gene expression. In particular, inducible expression in F1 plants obtained from crosses of driver and effector lines allows for rapid assessment of the cell type-specific impact of an effector with high temporal resolution. Thus, our comprehensive and flexible method is suitable for overcoming the limitations of ubiquitous genetic approaches, the outputs of which often are difficult to interpret due to the widespread existence of compensatory mechanisms and the integration of diverging effects in different cell types.
Pubmed
Web of science
Open Access
Yes
Create date
08/10/2018 7:40
Last modification date
20/08/2019 15:03
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