Ex vivo characterization of allo-MHC-restricted T cells specific for a single MHC-peptide complex.
Details
Serval ID
serval:BIB_9BC901FAC322
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ex vivo characterization of allo-MHC-restricted T cells specific for a single MHC-peptide complex.
Journal
Journal of immunology
ISSN
0022-1767
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
176
Number
4
Pages
2330-2336
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Alloreactive T cells are thought to be a potentially rich source of high-avidity T cells with therapeutic potential since tolerance to self-Ags is restricted to self-MHC recognition. Given the particularly high frequency of alloreactive T cells in the peripheral immune system, we used numerous MHC class I multimers to directly visualize and isolate viral and tumor Ag-specific alloreactive CD8 T cells. In fact, all but one specificities screened were undetectable in ex vivo labeling. In this study, we report the occurrence of CD8 T cells specifically labeled with allo-HLA-A*0201/Melan-A/MART-1(26-35) multimers at frequencies that are in the range of 10(-4) CD8 T cells and are thus detectable ex vivo by flow cytometry. We report the thymic generation and shaping of tumor Ag-specific, alloreactive T cells as well as their fate once seeded in the periphery. We show that these cells resemble their counterparts in HLA-A*0201-positive individuals, based on their structural and functional attributes.
Keywords
Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Child, Child, Preschool, Histocompatibility Antigens/immunology, Humans, Infant, Infant, Newborn, Isoantigens/immunology, Middle Aged, Molecular Sequence Data, Peptides/chemistry, Peptides/immunology, Phenotype, Receptors, Antigen, T-Cell, alpha-beta/chemistry, Receptors, Antigen, T-Cell, alpha-beta/immunology, Substrate Specificity, T-Lymphocytes/immunology, Transplantation, Homologous
Pubmed
Web of science
Create date
28/01/2008 12:13
Last modification date
20/08/2019 16:02