Ex vivo characterization of allo-MHC-restricted T cells specific for a single MHC-peptide complex.

Détails

ID Serval
serval:BIB_9BC901FAC322
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ex vivo characterization of allo-MHC-restricted T cells specific for a single MHC-peptide complex.
Périodique
Journal of immunology
Auteur(s)
Pittet M.J., Gati A., Le Gal F.A., Bioley G., Guillaume P., de Smedt M., Plum J., Speiser D.E., Cerottini J.C., Dietrich P.Y., Romero P., Zippelius A.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
176
Numéro
4
Pages
2330-2336
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Alloreactive T cells are thought to be a potentially rich source of high-avidity T cells with therapeutic potential since tolerance to self-Ags is restricted to self-MHC recognition. Given the particularly high frequency of alloreactive T cells in the peripheral immune system, we used numerous MHC class I multimers to directly visualize and isolate viral and tumor Ag-specific alloreactive CD8 T cells. In fact, all but one specificities screened were undetectable in ex vivo labeling. In this study, we report the occurrence of CD8 T cells specifically labeled with allo-HLA-A*0201/Melan-A/MART-1(26-35) multimers at frequencies that are in the range of 10(-4) CD8 T cells and are thus detectable ex vivo by flow cytometry. We report the thymic generation and shaping of tumor Ag-specific, alloreactive T cells as well as their fate once seeded in the periphery. We show that these cells resemble their counterparts in HLA-A*0201-positive individuals, based on their structural and functional attributes.
Mots-clé
Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Child, Child, Preschool, Histocompatibility Antigens/immunology, Humans, Infant, Infant, Newborn, Isoantigens/immunology, Middle Aged, Molecular Sequence Data, Peptides/chemistry, Peptides/immunology, Phenotype, Receptors, Antigen, T-Cell, alpha-beta/chemistry, Receptors, Antigen, T-Cell, alpha-beta/immunology, Substrate Specificity, T-Lymphocytes/immunology, Transplantation, Homologous
Pubmed
Web of science
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
20/08/2019 16:02
Données d'usage