Ex vivo characterization of allo-MHC-restricted T cells specific for a single MHC-peptide complex.

Details

Serval ID
serval:BIB_9BC901FAC322
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ex vivo characterization of allo-MHC-restricted T cells specific for a single MHC-peptide complex.
Journal
Journal of immunology
Author(s)
Pittet M.J., Gati A., Le Gal F.A., Bioley G., Guillaume P., de Smedt M., Plum J., Speiser D.E., Cerottini J.C., Dietrich P.Y., Romero P., Zippelius A.
ISSN
0022-1767
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
176
Number
4
Pages
2330-2336
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Alloreactive T cells are thought to be a potentially rich source of high-avidity T cells with therapeutic potential since tolerance to self-Ags is restricted to self-MHC recognition. Given the particularly high frequency of alloreactive T cells in the peripheral immune system, we used numerous MHC class I multimers to directly visualize and isolate viral and tumor Ag-specific alloreactive CD8 T cells. In fact, all but one specificities screened were undetectable in ex vivo labeling. In this study, we report the occurrence of CD8 T cells specifically labeled with allo-HLA-A*0201/Melan-A/MART-1(26-35) multimers at frequencies that are in the range of 10(-4) CD8 T cells and are thus detectable ex vivo by flow cytometry. We report the thymic generation and shaping of tumor Ag-specific, alloreactive T cells as well as their fate once seeded in the periphery. We show that these cells resemble their counterparts in HLA-A*0201-positive individuals, based on their structural and functional attributes.
Keywords
Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Child, Child, Preschool, Histocompatibility Antigens/immunology, Humans, Infant, Infant, Newborn, Isoantigens/immunology, Middle Aged, Molecular Sequence Data, Peptides/chemistry, Peptides/immunology, Phenotype, Receptors, Antigen, T-Cell, alpha-beta/chemistry, Receptors, Antigen, T-Cell, alpha-beta/immunology, Substrate Specificity, T-Lymphocytes/immunology, Transplantation, Homologous
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 15:02
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