HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation

Details

Serval ID
serval:BIB_8E3742B0C835
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation
Journal
Nature
Author(s)
Rosa A., Chande A., Ziglio S., De Sanctis V., Bertorelli R., Goh S. L., McCauley S. M., Nowosielska A., Antonarakis S. E., Luban J., Santoni F. A., Pizzato M.
ISSN
1476-4687 (Electronic)
ISSN-L
0028-0836
Publication state
Published
Issued date
2015
Volume
526
Number
7572
Pages
212-7
Language
english
Notes
Rosa, Annachiara
Chande, Ajit
Ziglio, Serena
De Sanctis, Veronica
Bertorelli, Roberto
Goh, Shih Lin
McCauley, Sean M
Nowosielska, Anetta
Antonarakis, Stylianos E
Luban, Jeremy
Santoni, Federico Andrea
Pizzato, Massimo
eng
249968/European Research Council/International
DP1 DA034990/DA/NIDA NIH HHS/
DP1DA034990/DA/NIDA NIH HHS/
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
England
Nature. 2015 Oct 8;526(7572):212-7. doi: 10.1038/nature15399. Epub 2015 Sep 30.
Abstract
HIV-1 Nef, a protein important for the development of AIDS, has well-characterized effects on host membrane trafficking and receptor downregulation. By an unidentified mechanism, Nef increases the intrinsic infectivity of HIV-1 virions in a host-cell-dependent manner. Here we identify the host transmembrane protein SERINC5, and to a lesser extent SERINC3, as a potent inhibitor of HIV-1 particle infectivity that is counteracted by Nef. SERINC5 localizes to the plasma membrane, where it is efficiently incorporated into budding HIV-1 virions and impairs subsequent virion penetration of susceptible target cells. Nef redirects SERINC5 to a Rab7-positive endosomal compartment and thereby excludes it from HIV-1 particles. The ability to counteract SERINC5 was conserved in Nef encoded by diverse primate immunodeficiency viruses, as well as in the structurally unrelated glycosylated Gag from murine leukaemia virus. These examples of functional conservation and convergent evolution emphasize the fundamental importance of SERINC5 as a potent anti-retroviral factor.
Keywords
Animals, Cell Line, Cell Membrane/metabolism/virology, Endosomes/chemistry/metabolism, Evolution, Molecular, Gene Products, gag/metabolism, Gene Products, nef/chemistry/metabolism, HIV-1/chemistry/*physiology, Host Specificity, *Host-Pathogen Interactions, Humans, Leukemia Virus, Murine/chemistry/physiology, Membrane Proteins/analysis/*antagonists & inhibitors/*metabolism, Neoplasm Proteins/metabolism, Primates/virology, Receptors, Cell Surface/metabolism, Virion/*chemistry/*metabolism, nef Gene Products, Human Immunodeficiency Virus/*metabolism, rab GTP-Binding Proteins/metabolism
Pubmed
Create date
20/05/2019 12:52
Last modification date
02/10/2019 5:26
Usage data