Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov
Details
Serval ID
serval:BIB_8CF81DC7C378
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov
Journal
Journal of Clinical Microbiology
ISSN
0095-1137 (Print)
Publication state
Published
Issued date
05/1996
Volume
34
Number
5
Pages
1100-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
A distinct group of slowly growing mycobacteria was identified on the basis of growth characteristics, biochemical and lipid profiles, and nucleic acid analyses. The isolates showed growth at 22 to 37 degrees C, yellow pigmentation, and negative tests for Tween 80 hydrolysis, nicotinic acid, nitrate reductase, and urease; tests for arylsulfatase, pyrazinamidase, and heat-stable catalase were variable. Analysis of cellular fatty acids by gas-liquid chromatography and mycolic acids by thin-layer chromatography and high-performance liquid chromatography indicated a distinctive pattern which was unlike those of other species. Determination of the 16S rRNA gene sequence showed a unique sequence closely related to Mycobacterium simiae and M. genavense. On the basis of DNA homology studies, we suggest that these organisms are representatives of a novel species, for which the name M. lentiflavum sp. nov. is proposed.
Keywords
Bacterial Typing Techniques
Base Sequence
DNA Primers/genetics
DNA, Bacterial/genetics
DNA, Ribosomal/genetics
Enzymes/analysis
Fatty Acids/analysis
Genes, Bacterial
Humans
Molecular Sequence Data
Mycobacterium/*classification/genetics/*isolation & purification
Phylogeny
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/genetics
Sequence Homology, Nucleic Acid
Species Specificity
Pubmed
Web of science
Create date
25/01/2008 15:46
Last modification date
20/08/2019 15:51