Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov

Détails

ID Serval
serval:BIB_8CF81DC7C378
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov
Périodique
Journal of Clinical Microbiology
Auteur(s)
Springer  B., Wu  W. K., Bodmer  T., Haase  G., Pfyffer  G. E., Kroppenstedt  R. M., Schroder  K. H., Emler  S., Kilburn  J. O., Kirschner  P., Telenti  A., Coyle  M. B., Bottger  E. C.
ISSN
0095-1137 (Print)
Statut éditorial
Publié
Date de publication
05/1996
Volume
34
Numéro
5
Pages
1100-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
A distinct group of slowly growing mycobacteria was identified on the basis of growth characteristics, biochemical and lipid profiles, and nucleic acid analyses. The isolates showed growth at 22 to 37 degrees C, yellow pigmentation, and negative tests for Tween 80 hydrolysis, nicotinic acid, nitrate reductase, and urease; tests for arylsulfatase, pyrazinamidase, and heat-stable catalase were variable. Analysis of cellular fatty acids by gas-liquid chromatography and mycolic acids by thin-layer chromatography and high-performance liquid chromatography indicated a distinctive pattern which was unlike those of other species. Determination of the 16S rRNA gene sequence showed a unique sequence closely related to Mycobacterium simiae and M. genavense. On the basis of DNA homology studies, we suggest that these organisms are representatives of a novel species, for which the name M. lentiflavum sp. nov. is proposed.
Mots-clé
Bacterial Typing Techniques Base Sequence DNA Primers/genetics DNA, Bacterial/genetics DNA, Ribosomal/genetics Enzymes/analysis Fatty Acids/analysis Genes, Bacterial Humans Molecular Sequence Data Mycobacterium/*classification/genetics/*isolation & purification Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Species Specificity
Pubmed
Web of science
Création de la notice
25/01/2008 15:46
Dernière modification de la notice
20/08/2019 15:51
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